منابع مشابه
Panhandle PCR strategy to amplify MLL genomic breakpoints in treatment-related leukemias.
Panhandle PCR amplifies genomic DNA with known 5' and unknown 3' sequences from a template with an intrastrand loop schematically shaped like a pan with a handle. We used panhandle PCR to clone MLL genomic breakpoints in two pediatric treatment-related leukemias. The karyotype in a case of treatment-related acute lymphoblastic leukemia showed the t(4;11)(q21;q23). Panhandle PCR amplified the tr...
متن کاملAmplification of 4-9-kb human genomic DNA flanking a known site using a panhandle PCR variant.
We present a method for the in vitro amplification of > 6.0 kb of DNA flanking a known site. This is accomplished by ligating an oligonucleotide to create an inverted repeat of a portion of the known sequence, followed by single-primer polymerase chain reaction (PCR) amplifications. This method generates a panhandle template following primer extension on the strand of interest. It does not invo...
متن کاملPanhandle and reverse-panhandle PCR enable cloning of der(11) and der(other) genomic breakpoint junctions of MLL translocations and identify complex translocation of MLL, AF-4, and CDK6.
We used panhandle PCR to clone the der(11) genomic breakpoint junction in three leukemias with t(4;11) and devised reverse-panhandle PCR to clone the breakpoint junction of the other derivative chromosome. This work contributes two elements to knowledge on MLL translocations. First is reverse-panhandle PCR for cloning breakpoint junctions of the other derivative chromosomes, sequences of which ...
متن کاملPanhandle Polymerase Chain Reaction Amplifies MLL Genomic Translocation
We used a new approach called panhandle polymerase chain sequence tags. MaLR sequences have not been found before near leukemia-associated translocation breakpoints. The reaction (PCR) to clone an MLL genomic translocation breakpoint in a case of acute lymphoblastic leukemia of innonrepetitive sequences were not homologous to known partner genes of MLL. Screening of somatic cell hybrid and fanc...
متن کاملDynamics of Land Use Change in the Florida Panhandle Using GIS Optimization Models: A Framework to Determine the Impact of Climate Change on the Florida Panhandle
The western “panhandle” region of Florida experienced greater development in the years 2000-2010 than the previous 40 years and greater than nearly all other parts of the United States. One reason is the fact that much of the coastal land in the peninsula of Florida is already developed whereas much of the Panhandle is empty. Another reason is the fact that increasing temperatures and less seve...
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ژورنال
عنوان ژورنال: Genome Research
سال: 1995
ISSN: 1088-9051
DOI: 10.1101/gr.4.5.s195